Generate BAM with barcodes from input raw FASTQ
Usage
BascetGetRaw(
bascetRoot,
rawmeta,
outputName = "debarcoded",
outputNameIncomplete = "incomplete_reads",
chemistry = "atrandi_wgs",
barcodeTolerance = NULL,
overwrite = FALSE,
runner = GetDefaultBascetRunner(),
bascet_instance = GetDefaultBascetInstance()
)
Arguments
- bascetRoot
The root folder where all Bascets are stored
- rawmeta
Metadata for the raw FASTQ input files. See DetectRawFileMeta
- outputName
Name output files: Debarcoded reads
- outputNameIncomplete
Name of output files: Reads that could not be parsed
- chemistry
The type of data to be parsed
- barcodeTolerance
Optional: Number of mismatches allowed in the barcode for it to still be considered valid
- overwrite
description
- runner
The job manager, specifying how the command will be run (e.g. locally, or via SLURM)
- bascet_instance
Configuration for how to run the Bascet Rust API